| Conditions | |
|---|---|
| Instrument | YL-9100 series (Young Lin Co. Ltd, Gyeonggi-do, Korea) |
| Column | YMC-Triart C18 (250×4.6 mm, 5 μm, YMC Co., Ltd., Kyoto, Japan) |
| Column temperature | 30°C |
| Elent | 0.1 M sodium phosphate buffer (pH 6.7) : acetonitrile (82:18) |
| Flow rate | 1 mL/min |
| Injection volume | 20 μL |
| Run time | 60 min |
| Detector | UV at 254 nm |
| Data calculator | Autochro-3000 (Young Lin) |