Table 2. Enzyme activity of Lactiplantibacillus pentosus LK013, Pediococcus pentosaceus LK019, Levilactobacillus brevis LK030, and Levilactobacillus brevis P215 using API ZYM kit

Substrats	LK013	LK019	LK030	P215
Esterase (C4)	w3)	-	+	+
Esterase lipase (C8)	w	-	+	+
Lipase (C14)	+2)	-	-	-
Leucine arylamidase	+	+++1)	+	+
Valine arylamidase	+	+++	+	+
Cystine arylamidase	w	+	w	+
Acid phosphatase	w	+	+	w
Naphthol-AS-BI-phosphohydrolase	+	++1)	+	+
α-Galactosidase	w	-	+	+
β-Glucuronidase	+	-	+	+
β-Glucosidase	-4)	-	+	w
α-Glucosidase	+	-	+	+
β-Glucosidase	+	++	+	+
N-Acetyl-β-glucosaminidase	+	++	-	-

++ and +++, strong positive;
+, positive;
w, weak positive;
-, negative.
All of the strains are negative for alkaline phosphatase, trypsin, α-chymotrypsin, α-mannosidase, and α-fucosidase.