발효김치 중 면역활성 젖산균의 분리 및 특성

최재임^†, 강승범^†, 박진호, 유성호, 윤성식^*

Isolation and Characterization of an Immune-Stimulating Lactic Acid Bacteria from Fermenting kimchi

Jea-Im Choi^†, Seung-Bum Kang^†, Jin-Ho Park, Sung-Ho Yoo, Sung-Sik Yoon^*

연세대학교 생명과학기술학부

Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Wonju 220-710, Korea

Equally contributed authors.

^*Corresponding author: Sung-Sik Yoon, Division of Biological Science and Technology, Yonsei University, Wonju 220-710, Korea. Tel: 82-33-760-2251, Fax: 82-33-760-5576, E-mail: sungsik@yonsei.ac.kr

Copyright © Korean Society for Lactic Acid Bacteria. All rights reserved. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jun 30, 2019

Abstract

Isolation of Lactobacillus plantarum FBT215 (L. plantarum FBT215) from fermenting kimchi, which has immunostimulatory effects, was characterized in this study. To isolate probiotic lactic acid bacteria (LAB) from kimchi, properly diluted kimchi samples were spread on MRS agar incorporated BCP and sodium azide. One hundred fifty and four LAB isolates were isolated, screened for immune-stimulatory activities in murine RAW 264.7 cells. Among the 24 LAB strains after MTT assay, FBT215 has shown the best cytokine activity of expression of 676.9±22.2 pg/mL of interleukin IL-1α and 845.2±31.6 pg/mL of TNF-α. For identification, the typing experiments using 16S rDNA sequencing and API 50 CH kit confirmed that the FBT215 belongs to Lactobacillus plantarum, shortly called as L. plantarum FBT215. The FBT215 strain showed high antibiotic susceptibility, weak acid tolerance, bile salt tolerance and BSH activity but did not produce bacteriocin against indicator strains tested. API ZYM kit analysis resulted that the L. plantarum FBT215 produces seven different kinds of enzymes. In conclusion, these properties would be used as a functional probiotic culture in the manufacture of fermented foods.

Keywords: Lactobacillus plantarum; immuno-stimulation; cytokine; TNF-α; IL-1α; BSH activity