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Korean Society for Lactic Acid Bacteria and Probiotics
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Lactobacillus plantarum L67의 항알러지 활성

신미영1, 송수연1, 고종호2, 김광현1, 오세종1,*
Miyoung Shin1, Sooyeon Song1, Jongho Koh2, Kwang-Hyun Kim1, Sejong Oh1,*
1전남대학교 동물자원학부
2한국폴리텍특성화대학
1Division of Animal Science, Chonnam National University, Gwangju 61186, Korea
2Department of Bio-Food Processing & Analysis, Bio-Campus, Korea Polytechnic College, Nonsan-si 32943, Korea
*Corresponding author: Sejong Oh, Division of Anima; Science, Chonnam National University, 62286, Republic of Korea. Tel: +82-62-530-2116 Fax: +82-62-530-2129, E-mail: soh@jnu.ac.kr

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Published Online: Jun 30, 2019

Abstract

In this study, we found that the glycoprotein (18 kDa) isolated from Lactobacillus plantarum L67 (L67 protein) inhibits allergy-related inflammatory mediators in BPA treated rat basophilic leukemia 2H3 (RBL-2H3) cells and primary cultured splenocytes. First, we measured the levels of ERK, p38, and AP-1 by Western blotting and we evaluated the expression of IL-1β, IL-6, and IL-10 cytokines by real-time quantitative PCR. Finally, we evaluated the applicability of L. plantarum L67 as a yogurt starter culture by measuring pH, enumeration of bacteria, and sensory scores. The results of this study shows that L67 protein inhibits the phosphorylation of ERK and p38 MAPK, as well as the activation of AP-1 (c-Fos and c-Jun). With inflammationrelated factors (Th2-related cytokines), L67 protein inhibits BPA-induced IL-1β, IL-6, and IL-10 activity. Bisphenol A stimulates MAPK signaling, which induces increased GATA-3 expression, and GATA-3 induces the differentiation of Th2 cells and secretion of Th2-related cytokines. Our findings showed that L67 protein may regulate the activation of GATA3 and T-bet through the induction of MAPK signaling, and it relieves the Th1/Th2 cytokine profile imbalance. Taken together, these findings demonstrate that the L. plantarum L67 strain is a potential food supplement and starter culture, that could help in the prevention of Th2-related immune disorders.

Keywords: allergy; bisphenol A; Lactobacillus plantarum L67; probiotics