Article

한국인 모유영양아로부터 분리한 Enterococcus faecium ID9201의 콜레스테롤 저하효과

이승훈, 김영후, 김민구, 강재훈, 강대중*
Seung-Hun Lee, Young-Hoo Kim, Min-Goo Kim, Jae-Hoon Kang, Dae-Jung Kang*
Author Information & Copyright
일동제약(주) 중앙연구소
Bioprocess Engineering Team, Research Laboratories, ILDONG pharmaceutical Co., Ltd., Hwaseong 445-170, Korea
*Corresponding author: Dae-Jung Kang, Bioprocess Engineering Team, Research Laboratories, ILDONG pharmaceutical Co., Ltd., Hwaseong 445-170, Korea, Tel: 82-31-371-2881, Fax: 82-31-371-2900, E-mail: djkang@ildong.com

Copyright © Korean Society for Lactic Acid Bacteria. All rights reserved. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jun 30, 2019

Abstract

We selected lactic acid bacteria to reduce cholesterol levels in vitro and in vivo. Among the 140 strains tested, a strain ID9201 was shown to reduce cholesterol in the spent medium over 30% and to increase cholesterol in cell pellet. A strain ID9201 isolated from breast milk-fed Korean infant feces was identified as Enterococcus faecium on the basis of morphological analysis, biochemical analysis (API kit), and 16S rDNA sequence analysis. A freeze-dried powder of the strain was fed to Sprague-Dawley(SD) rats with high cholesterol diet for 6 wk. In the control group fed cholesterol enriched diet without probiotics, the serum cholesterol increased by 1.9 times in comparison with the initial value. Among the test group, ID9201 group showed the lowest cholesterol increase, 1.5 times of initial serum cholesterol, as in the blank group fed normal diet without probiotics. Based on these data, we assumed that ID9201 inhibited intestinal absorption of dietary cholesterol.

Keywords: Enterococcus faecium ID9201; cholesterol; conjugated bile acid; deconjugated bile acid; bile salt hydrolase