김치 및 유아분변에서 분리한 유산균의 Probiotic 활성 조사

송수연1, 이현준2, 김종훈2, 오세종1,*
Sooyeon Song1, Hyun-Jun Lee2, Jong-Hoon Kim2, Sejong Oh1,*
Author Information & Copyright
1전남대학교 동물자원학부
2㈜농심 연구소
1Division of Animal Science, Chonnam National University, Gwangju 500-757, Korea
2Research & Development Center, Nong Shim Co. Ltd., Seoul 156-709, Korea
*Corresponding author: Sejong Oh, Division of Animal Science, Chonnam National University, Gwangju 61186, Korea Tel: 82-62-530-2116, Fax: 82-62-530-2129, E-mail:

Copyright © Korean Society for Lactic Acid Bacteria. All rights reserved. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Published Online: Jun 30, 2019


We isolated 105 strains of lactic acid bacteria (LAB) from home-made kimchi and breast milk-fed Korean infant feces on the basis of morphological analysis. This study aimed to compare the probiotic characteristics of selected strains of LAB including bile and acid tolerances, cholesterol assimilation and adhesion activity. Among the isolates of LAB, 54 isolates were identified as Lactobacillus plantarum (14 strains), L. brevis (12 strains), L. sakei (9 strains), L. acidophilus (3 strains), L. casei (1 strain), and L. fermentum (1 strain). Acid tolerances under artificial gastric juice, pH 2.5 for 2 h at 37°C, were significantly different among the Lactobacillus species. Lactobacillus acidophilus and L. plantarum strains exhibited high tolerance in acid and bile. Lactobacillus acidophilus strains exhibited high cholesterol assimilation activity and showed a significantly higher tolerance to 0.3% bile acid than other Lactobacillus strains (p<0.05). Based on these results, we selected the best strain, named NS1 (L. acidophilus) as a potential probiotics that can be utilized in the manufacturing of dairy foods and dietary supplements.

Keywords: Lactobacillus acidophilus; probiotics; cholesterol; bile tolerance; acid tolerance