케피어 유래 박테리오신 생산 Enterococcus faecium 균주 선발

A bacteriocin producing lactic acid bacterium was isolated from kefir which is functional fermented milk. The CJNU 2008 strain was identified as Enterococcus faecium by 16S rRNA gene sequence analysis and the strain named E. faecium CJNU 2008. Antibacterial spectrum of E. faecium CJNU 2008 has shown that several strains including pathogenic bacteria Listeria monocytogenes and Staphylococcus aureus were inhibited by the cell and culture supernatant. Optimal culture conditions for production of the bacteriocin from E. faecium CJNU 2008 were MRS broth as a medium, 30°C, and pH 7.0.